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Norman Kuo

3rd

Previous positions

  • Bioinformatic Graduate Research Assistant at Laboratory for Pathogenesis of Clinical Drug Resistance and Persistence
  • Lab Technician II at Molecular Diagnostic Services. Inc

Education

San Diego State University-California State University, Master’s degree, Bioinformatics

172

Background

Summary

A highly skilled bioinformatics analyst specialized in data collection, analysis and management. Works well under pressure, with a commitment to maintaining accurate documentation, able to multi-task and prioritize to meet deadlines.

Experience

  • Software Test Engineer

    Illumina

    June 2018 – Present(1 year 4 months)Greater San Diego Area

    – Work with software developers, testers and bioinformatics scientist in designing, performing, and improving verification and validation tests throughout the software development life cycle to ensure a high-quality software product. – Identifying, analysing, and removing the bugs or defect from the software code that cause the software to behave in an unintended way, produce incorrect result, or break the requirement. – Prepare necessary test deliverables required for formal software/product release, including Software Verification Plans, Test Protocols, Test Reports, Software User Guide, Deployment Plan. – Develop and maintain automated testing script (using Python, Bash scripts, Docker and Jenkins) – Executing Pipeline workflow under different environment (Docker, Singularity, AWS EC2, Cromwell/WDL) – – Work closely with the team and the product owners and follow Agile/Scrum practice to ensure software quality

  • – Systematic literature review for functional curation of H37Rv proteins product of genes for which annotation is lacking and develop innovative annotation methodology on Mycobacterium tuberculosis reference genome (H37Rv) through combination of in silico and manual gene curation. – Quality control on de novo assembly Mycobacterium tuberculosis clinical isolates to ensure assembly is correct – Predict DNA motif pattern and recognize promoter region of Mycobacterium tuberculosis using Convolutional Neural Network (Python: Keras TensorFlow backend) on Next Generation Sequencing (NGS) clinical isolates data – Assembled and implemented back-end and front-end of Mycobacterium Tuberculosis MySQL database, with a team of developers using PHP, HTML, CSS, WAMP server, Docker, and SQL. – Use python and bash to parse, extract, and manipulate different types of machine readable data. – Applied statistical analysis methods to optimize process and data quality using Python and R

  • Lab Technician II

    Molecular Diagnostic Services. Inc

    August 2011 – August 2016(5 years)Greater San Diego Area

    – Managing the laboratory, with responsibility for conducting lab checks, obtaining quotes, managing documentation, receiving and processing samples, and organizing supplies and specimens for all projects. – Executing experiments to assist with the development of vaccines, antibodies, and virus’ utilizing aseptic tissue culture techniques. – Performing a range of laboratory techniques, including PCR, Cell Culture, Karyotyping, FISH analysis, radioactive processes RIA, Androgen Receptor Competitive Binding Assays, inoculating for sterility and B&F testing for various products, completing direct, competitive and sandwich ELISA techniques, and general laboratory practices including buffer, solutions, and cell culture media. – Designing, managing websites, troubleshooting computers, resolving issues with emails and website hosting.

Education

  • San Diego State University-California State University

    Master’s degree, Bioinformatics

    2016 – 2018

    Thesis Project: Create and optimize Pan-genome analysis pipeline on Next Generation Sequencing de novo assembled Mycobacterium tuberculosis (Mtb) clinical isolates strains in hoping to identify possible correlation between genes presented in Mycobacterium tuberculosis clinical isolates and Mycobacterium tuberculosis drug resistance.

  • University of California, San Diego

    Bachelor of Science (BS), Molecular Biology

    2007 – 2011

Languages

  • Chinese

    Native or bilingual proficiency

  • English

    Native or bilingual proficiency

  • Japanese

    Limited working proficiency

Skills & Expertise

  • Training
  • Laboratory Skills
  • Leadership
  • CSS
  • Bioinformatics
  • Data Analysis
  • Database Design
  • Manufacturing
  • Polymerase Chain Reaction (PCR)
  • Annotation
  • Data Mining
  • Machine Learning
  • Research
  • Management
  • PCR
  • Structural Bioinformatics
  • PHP
  • Data Entry
  • Karyotyping
  • python
  • Python (Programming Language)
  • ELISA
  • Pattern Recognition
  • MySQL
  • HTML
  • R
  • Dna Sequencing
  • Cell Culture